22/07/2017
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Agriculture and Natural Resources>Medicinal Plants>
Medicinal Plants Biotechnology

 The use of biotechnological tools in medicinal plants science is very limited as compared to other crops. However, in recent years the engineering of agronomic traits in medicinal plants led to considerable developments.
Institute of Medicinal Plants uses Biotechnology as an important tool in order to select, multiply and preserve the critical genotypes of medicinal plants. Some of these activities include: in vitro propagation, organ culture, cell suspension culture, secondary metabolite production, gene transformation, cytogenetic and molecular marker studies. In ACECR protocols for propagation, embryogenesis and regeneration of Aloe vera, Taxus bacata, Corylus avellana, Ferula assa foetida and gene transformation of papaver somniferum, Genetic Diversity  of Hyosyamus, Artimisia, Alcea, Anethum, karyotype analysis of Frula and Ducrosia, and also engineering of secondary metabolites and somaclonal variation of Ducrosia have been developed. 
 

1) Laboratory organization
Phytotron
Culture room
Acrylamide room
Incubator
Centrifuge
Weighing balance
Autoclave
Vacuum pump
Hat platecum magnetic stirrer
Refrigerator -80
pH meter
Light Microscope


2) Tissue culture:
Preparation of sterile media, plant material and small instruments
Isolation
Incubation
Sub-culturing

3) Gene transformation
Optimization of somatic embryogenesis
Cutting and joining DNA molecules
Cloning vectors
cDNA and Genomic cloning and analysis of cloned DNA sequences

4)  Molecular Markers
DNA extraction
RAPD
AFLP

5)  Cytogenetic
Germination
Fixation
Hybridization
Staining
Squashing





 






 

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The ACECR affiliate in charge:
Institute of Medicinal Plants
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