18/11/2017
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Agriculture and Natural Resources>Medicinal Plants>
Agriculture and Natural Resources>Sapling, Grafting and Seed>
Health, Biology and Medicine>Herbal Medicine >
Health, Biology and Medicine>Public Health>
Micropropagation of Aloe vera via Tissue Culture



Advantages:

1-    Rapid propagation in limited time and space; we increase speed of propagation of Aloe vera more than 50-80 times.

2-      Virus-free

3-      Without somaclonal variation

      4-   Reasonable price
 
 we are able to provide mor than 300000 sprigs of Aloe vera annually
 
Process Flowchart:
 
 
  Callus induction and somatic embryogenesis:
    After cutting explants into suitable size (2-3cm),explants are transferred to culture bottles containing MS medium with  BA and  IBA.
   
 
 
 
  Direct shoot:
 
 
   Proliferation:
    After one month of culture period of the explants with newly form shoots were taken out under strict aseptic conditions and were excised from the parent plant with help of sterile scalpel blade and sterile forceps and inoculated into new bottles containing solid and liquid MS basal medium with different set of growth hormones.
 
   
 
 
  Root induction:
 
  Root induction:
    Microshoots showing rooting after 15 days of culture.
 
  Adaptation:
    After 15 days of culture on rooting media, the plantlets were shifted to plasticpots for their hardening prior to final transfer to soil to natural conditions.
 
   Adaptation in green house:
   Plantlets that were transferred to the plastic pots in polyhouse showed good percentage of survival of 85% .After keeping plantlets for initial ten days in polyhouse, the plantlets were transferred to shade house under less humidity and temperature controlled conditions and indirect sunlight.
    Elite Plant Material of Aloe vera Growing in the green house.
   
 
Our offerings :
Finished Product
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Training
Development of Training Courses

The ACECR affiliate in charge:
Institute of Medicinal Plants
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